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Valve-through tire puncture sealant compositions including dissolved and undissolved natural bio-materials, anti-freezing agents and other rigid particles are invented. In the preparation, an aqueous solution with 0.1-10 wt. % dissolved bio-materials and 0.1 – 10 wt. % tackifier is mixed with anti-freezing agent, in which 0.01-5 wt. % rigid particles of bio-materials and other particles are...